|
KMID : 1035420230110040125
|
|
Journal of The Korean Society of Integrative Medicine
2023 Volume.11 No. 4 p.125 ~ p.133
|
|
|
Antibacterial and Growth Inhibitory Effects of Liriope Platyphylla Ethanol Extract on Streptococcus Mutnas and Porphyromonas Gingivalis
|
|
Chun Su-Hyeon
Park Ju-Yeon
Lee Hyeon-Ji
Jeong Ji-Eun
Cha Eun-Suk
Park Chung-Mu
Yoon Hyun-Seo
|
|
|
Abstract
|
|
|
Purpose : This study aimed to analyze the antibacterial activity of Liriope platyphylla ethanol extract (LPEE) against Streptococcus mutans and Porphyromonas gingivalis and to validate its potential for the prevention and treatment of dental caries, gingivitis, and periodontal disease.
Methods : To verify the antibacterial effect of L. pulsatilla ethanolic extract (LPEE) against S. mutans and P. gingivalis, the disk diffusion method was used to determine the inhibition zones at concentrations of 50, 100, 200, and 300 ㎎/㎖. To determine the minimum inhibition concentration (MIC), the final dose of LPEE was .2, .4, .8, 1.6, 2.5, and 5.0 ㎎/㎖, and the minimum bactericidal concentration (MBC) was determined based on the MIC results. To confirm the growth inhibitory effect of LPEE on both pathogens, the absorbance was measured at 600 nm after each incubation for 0, 3, 6, 12, and 24 hr at concentrations of .8, 1.6, 2.5, and 5.0 ㎎/㎖.
Results : The cytotoxicity of LPEE was evaluated and the cell viability was more than 70 % at 400 ㎎/㎖. Therefore, concentrations of 50, 100, 200, and 300 ㎎/㎖ were used in this study. The antimicrobial effect against S. mutans was seen at 100 ㎎/㎖ and grew in a concentration-dependent manner, while P. gingivalis was effective at 50 ㎎/㎖ with the dose dependency. The MIC was .8 ㎎/㎖ for both strains, and the MBC was 1.6 ㎎/㎖ with the same results. The growth inhibitory effect of LPEE on S. mutans and P. gingivalis was observed, even at low concentrations.
Conclusion : The antibacterial effect of LPEE was evaluated through the analysis of MIC, MBC, and growth inhibition effect on S. mutans and P. gingivalis, which suggests LPEE might have the possibility of utilization as a preventive and therapeutic composition for oral diseases.
|
|
|
KEYWORD
|
|
|
antibacterial, growth inhibition, liriope platyphylla ethanol extract, porphyromonas gingivalis, streptococcus mutnas
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|